Agrobacterium rhizogenes–induced Altered Morphology and Physiology in Rubber Dandelion after Genetic Transformation
نویسندگان
چکیده
Agrobacterium rhizogenes transformation is a more rapid method of obtaining transgenic and edited rubber dandelion ( Taraxacum kok-saghyz ) plants than tumefaciens . The hairy root rol genes are present alongside transgenes after transformation, they change the morphology significantly. Although these useful visual markers indicating successful dandelion, modify phenotype induced by target ultimately detrimental to agronomic traits. Fortunately, can be removed conventional plant breeding because segregate in progeny separately from targeted transgenes. However, it preferable have preliminary identification promising effects or gene edits before removal so that only best used for breeding. Therefore, goal this research was characterize – + that, future, transgene easily distinguished transgene– plants. This requires gene–induced morphological changes simply assayed physiological traits first characterized thoroughly may observed. Taproot formation reduced eliminated genes, -induced roots identifiable grow shallowly potting soil, partial unearthing needed. Both leaf flower numbers increased but leaves flowers smaller wild type with longer stalks. rosette doming caused induction large number primordia obvious rooted as young 1 month old. Photosynthetic rates significantly plants, although growth not. An accurate description A. allow initial selection transformed confirmation polymerase chain reaction, phenotypic comparison expressing gene, those gene.
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ژورنال
عنوان ژورنال: Journal of the American Society for Horticultural Science
سال: 2023
ISSN: ['0003-1062', '2327-9788']
DOI: https://doi.org/10.21273/jashs05217-22